Real-time measurement and analysis of translational and rotational speeds of moving objects in microscope fields

Coltelli P., Evangelisti M., Evangelista V., Gualtieri P.

I.4.1 Digitization and Image Capture  I.4.7 Feature Measurement  92C55 Biomedical imaging and signal processing  Automatic velocity determination  Image sequence analysis 

This paper describes a digital system designed for the automatic detection and measurement of the velocity of moving objects in images acquired by means of a common TV-camera mounted onto a microscope. The main characteristics of this system are the following: 1) it can perform a realtime gray level difference between two successive frames in order to detect moving objects and to suppress stationary objects (subtraction procedure); usually the delay between two successive frames varies linearly from 40 msec to 1920 msec; 2) it reduces the size of images resulting from the subtraction procedure (difference images) and stores them in the frame memory; the result of these operation, all performed in real-time, is a film of time sequences; 3) it performs an automatic labelization in order to recognize the moving microorganisms and to calculate their area in each difference image; 4) it calculates and plots the variation of the average area of the cells moving in the microscope field; 5) it completes the analysis in few seconds.

Source: Lecture notes in computer science 4826 (2007): 128–135.

Publisher: Springer, Berlin , Germania