An image processing procedure for the assessment of normality curves of motility of human granulocytes in micropore filters
Azzarà A, Chimenti M, Carulli G, Rizzutigullaci A, Ambrogi F
PMN Image analysis Life and medical sciences Image processing and computer vision Migration curves Clinical Biochemistry General Medicine Chemotaxis
With the conventional methods used in current practice, the results of human granulocyte motility assays are expressed by a numerical value that defines the leading front of cells into micropore filters. The authors have developed a fast procedure which allows the complete curve of polymorphonuclear leukocyte (PMN) migration to be obtained, from the initial plane of a filter to the maximum propagation depth. In this way, more information is given than that expressed by a single parameter. The paper describes the procedure used to determine the normality curves both for random and stimulated migration, according to a simple model which defines the PMN propagation in micropore filters. A normality band that defines variations due to the stochastic process is also reported. © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Source: SCANDINAVIAN JOURNAL OF CLINICAL AND LABORATORY INVESTIGATION, vol. 55 (issue 5), pp. 399-408
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@article{oai:it.cnr:prodotti:410520,
title = {An image processing procedure for the assessment of normality curves of motility of human granulocytes in micropore filters},
author = {Azzarà A and Chimenti M and Carulli G and Rizzutigullaci A and Ambrogi F},
doi = {10.3109/00365519509104979},
year = {1995}
}