Ricostruzione tridimensionale della struttura della cromatina da dati tipo Chromosome Conformation Capture. Nota riservata, progetto InterOmics

Caudai C

Epigenetics  3D chromatin structure  Chromosome conformation capture  J.3 LIFE AND MEDICAL SCIENCES.  

This document reports part of the WP1-ISTI unit activity in the framework of the national Flagship Project InterOmics, on the 3D chromatin structure analysis from Hi-C experiments. Compared with other methods presented in the literature, our approach uses a new solution model incorporating sound prior knowledge, and a new reconstruction criterion that does not require an explicit translation of the Hi-C data into Euclidean distances between pairs of genomic loci. This approach also allows us to solve the problem in a multiscale setting, by reconstructing significant fragments separately at high resolution and then putting them together through the same criterion applied to lower-resolution data.